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Influenza Propagation Quantification And Storage Pdf

influenza propagation quantification and storage pdf

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Influenza: propagation, quantification, and storage

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Skip to main content Skip to table of contents. Advertisement Hide. This service is more advanced with JavaScript available. Influenza Virus Methods and Protocols. About About this book Protocols Table of contents 30 protocols About this book Introduction This book provides researchers with widely used techniques for the study of virology, focusing on molecular biology and imaging to encourage mechanistic investigation of virus-host interactions.

Influenza virus propagation in embryonated chicken eggs.

Skip to main content Skip to table of contents. Advertisement Hide. This service is more advanced with JavaScript available. Influenza Virus Methods and Protocols. Front Matter Pages i-xvi. Understanding Influenza. Pages

Metrics details. The infectivity of influenza A viruses can differ among the various primary cells and continuous cell lines used for such measurements. The infectivity of influenza viruses can differ among the various primary cells and continuous cell lines used for such measurements [ 1 , 2 ]. As the term "infectivity" has many meanings in virology, in this manuscript, infectivity is broadly defined as the ability of a virus particle to enter a host cell and form viable progeny virions. Accurate viable virus counts are essential for inhalation exposure studies with aerosolized viruses [ 3 ], for correlation of viable count to genome equivalence in level of detection studies, and other relevant work with influenza viruses. Quantitative RT-PCR methods are not suitable, as they do not distinguish between viable and non-viable virus particles.

Influenza Virus

Influenza viruses are negative-sense, single-stranded, enveloped RNA viruses belonging to the family Orthomyxoviridae. Three types exist, influenza A, B, and C. All infect humans, but only A and B are major human pathogens. Influenza type A viruses are divided into subtypes based on genetic and antigenic differences in the two surface spike proteins, hemagglutinin HA and neuraminidase NA.

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Influenza: propagation, quantification, and storage

Influenza virus infections are responsible for more than , deaths annually. Influenza virus isolation, propagation, and characterization protocols are critical for completing reproducible basic research studies and for generating vaccine seed stocks. Detailed protocols for the isolation and identification of influenza virus have been recently reported Eisfeld et al. However, there are few standardized protocols focused on the propagation and characterization of viral isolates, and as a result, viruses propagated in different conditions in different laboratories often have distinct in vitro and in vivo characteristics.

Influenza viruses are negative-sense, single-stranded, enveloped RNA viruses belonging to the family Orthomyxoviridae. Three types exist, influenza A, B, and C. All infect humans, but only A and B are major human pathogens. Influenza type A viruses are divided into subtypes based on genetic and antigenic differences in the two surface spike proteins, hemagglutinin HA and neuraminidase NA. The appropriate cell lines to be used for isolation of influenza A or B viruses depend on the clinical information and the host of origin.

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    18.06.2021 at 12:25
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    Influenza: propagation, quantification, and storage. Curr Protoc Microbiol. Dec;Chapter Unit 15G doi: /mc15g01s3.

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    Influenza: Propagation, Quantification, and Storage National Institutes of Health (mydowntownsmyrna.org).

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